Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Mislocalisation of FLT3-ITD receptor contributes to MV4-11 leukaemia cell resistance to antibody-drug conjugate

doi: 10.1080/14756366.2026.2638027

Figure Lengend Snippet: The surface expression of FLT3 receptor in MV4-11 (FLT3-ITD) and THP-1 (FLT3-wt) cells compared to K562 (FLT3-neg). Theexpression of FLT3 on the surface of both cells was determined by flow cytometry. The histograms showed FLT3 mAb bound to FLT3 receptors on both mutant and wild cell lines.

Article Snippet: MV4-11 cells (CRL-9591 TM ), KG-1a cells (CCL-246.1 TM ), K562 cells (CCL-243 TM ), THP-1 cells (TIB-202 TM ) and U937 cells (CRL-1593.2 TM ) were purchased from the American Type Culture Collection (Manassas, VA, US).

Techniques: Expressing, Flow Cytometry, Mutagenesis

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Mislocalisation of FLT3-ITD receptor contributes to MV4-11 leukaemia cell resistance to antibody-drug conjugate

doi: 10.1080/14756366.2026.2638027

Figure Lengend Snippet: Temperature-dependent internalisation of FLT3 mAb in different FLT3-expressing cells. Confocal fluorescence images showing FLT3 mAb (green) localisation in (a) K562 (FLT3-negative), (b) THP-1 (FLT3-wt), and (c) MV4-11 (FLT3-ITD) cells incubated at 4 °C or 37 °C. Nuclei were stained with DAPI (blue), and plasma membranes were labelled with WGA (magenta). No fluorescent signal of FLT3 mAb was observed in K562 cells at either temperature, while both THP-1 and MV4-11 cells showed strong fluorescent accumulation of FLT3 mAb inside the cells at 37 °C, indicating temperature-dependent internalisation through receptor-mediated endocytosis.

Article Snippet: MV4-11 cells (CRL-9591 TM ), KG-1a cells (CCL-246.1 TM ), K562 cells (CCL-243 TM ), THP-1 cells (TIB-202 TM ) and U937 cells (CRL-1593.2 TM ) were purchased from the American Type Culture Collection (Manassas, VA, US).

Techniques: Expressing, Fluorescence, Incubation, Staining, Clinical Proteomics

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Mislocalisation of FLT3-ITD receptor contributes to MV4-11 leukaemia cell resistance to antibody-drug conjugate

doi: 10.1080/14756366.2026.2638027

Figure Lengend Snippet: The effects of various conditions on binding and internalisation of FLT3 mAb by FLT3 receptors on MV4-11 cells as observed using flow cytometry. (a) The incubation time influences the uptake of mAb into MV4-11 cells at 37 °C, while the incubation time did not affect the cellular uptake at 4 °C. (b) The effects of various concentrations of FLT3 mAb on FLT3 receptor binding were evaluated at 4 °C. (c) The receptor recycling inhibitors such as monensin, brefeldin A, and the cocktail did not have any effect on FLT3 internalisation 4 °C. (d) Monensin, brefeldin A, and the cocktail significantly inhibited the internalisation of FLT3 receptors at 37 °C. (e) ATP depleting agents (NaN 3 and 2-Deoxy-D-glucose) did not have any impact on the internalisation of the receptors at 4 °C. (f) NaN 3 and 2-Deoxy-D-glucose as ATP inhibitors significantly suppress the uptake of FLT3 mAb into MV4-11 cells at 37 °C. The data showed mean ± SD in three independent experiments. The statistical differences were determined using one-way ANOVA and were considered significant at **** p < 0.0001.

Article Snippet: MV4-11 cells (CRL-9591 TM ), KG-1a cells (CCL-246.1 TM ), K562 cells (CCL-243 TM ), THP-1 cells (TIB-202 TM ) and U937 cells (CRL-1593.2 TM ) were purchased from the American Type Culture Collection (Manassas, VA, US).

Techniques: Binding Assay, Flow Cytometry, Incubation

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Mislocalisation of FLT3-ITD receptor contributes to MV4-11 leukaemia cell resistance to antibody-drug conjugate

doi: 10.1080/14756366.2026.2638027

Figure Lengend Snippet: Differential localisation pathways of FLT3 mAb (green) in (a) FLT3-ITD MV4-11 and (b) FLT3-wt THP-1 cells. The cells were incubated with FLT3-AF488 mAb (green) at 37 °C for 1 h. Cellular organelles were stained with LysoTracker ™ (red, lysosomal marker), GM130 (magenta, Golgi marker), and DAPI (blue, nuclear marker). In MV4-11 cells, FLT3 predominantly co-localised with the Golgi marker (white arrow), indicating accumulation in the Golgi region. In contrast, THP-1 cells showed strong co-localisation of FLT3 with the lysosomal marker (yellow arrow), suggesting normal receptor trafficking through the endosomal-lysosomal pathway.

Article Snippet: MV4-11 cells (CRL-9591 TM ), KG-1a cells (CCL-246.1 TM ), K562 cells (CCL-243 TM ), THP-1 cells (TIB-202 TM ) and U937 cells (CRL-1593.2 TM ) were purchased from the American Type Culture Collection (Manassas, VA, US).

Techniques: Incubation, Staining, Marker

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Mislocalisation of FLT3-ITD receptor contributes to MV4-11 leukaemia cell resistance to antibody-drug conjugate

doi: 10.1080/14756366.2026.2638027

Figure Lengend Snippet: Cytotoxicity of FLT3-MMAE conjugate (green), DBCO-MMAE (red) and MMAE (blue) in (a) MV4-11 (FLT3-ITD), (b) THP-1 (FLT3-wt), and (c) K562 (FLT3-neg) cells after 96 h incubation. Cell viability was determined using the MTT assay. (d) Comparison of IC 50 values of the FLT3-MMAE conjugate in MV4-11 and THP-1 cells, indicating significantly higher cytotoxicity in FLT3-wt THP-1 cells compared with FLT3-ITD MV4-11 ( p < 0.01). The data represented mean ± SD from triplicate independent experiments.

Article Snippet: MV4-11 cells (CRL-9591 TM ), KG-1a cells (CCL-246.1 TM ), K562 cells (CCL-243 TM ), THP-1 cells (TIB-202 TM ) and U937 cells (CRL-1593.2 TM ) were purchased from the American Type Culture Collection (Manassas, VA, US).

Techniques: Incubation, MTT Assay, Comparison

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Mislocalisation of FLT3-ITD receptor contributes to MV4-11 leukaemia cell resistance to antibody-drug conjugate

doi: 10.1080/14756366.2026.2638027

Figure Lengend Snippet: Lysosomal processing of FLT3-MMAE conjugate (1 µM) in (a) MV4-11 (FLT3-ITD) and (b) THP-1 (FLT3-wt) cells in the presence of bafilomycin A1 (Baf A1) (0–1 nM). Data represented mean ± SD from three independent experiments. Statistical analysis was performed using one-way ANOVA, with significance defined as *** p < 0.001 and **** p < 0.0001.

Article Snippet: MV4-11 cells (CRL-9591 TM ), KG-1a cells (CCL-246.1 TM ), K562 cells (CCL-243 TM ), THP-1 cells (TIB-202 TM ) and U937 cells (CRL-1593.2 TM ) were purchased from the American Type Culture Collection (Manassas, VA, US).

Techniques:

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: MV411 cells express C-type lectin-like molecule-1 (CLL1). A) Confocal microscopy of MV411 cells stained for DAPI, CLL1, and actin. Scale bar = 3 μm. B) Histogram showing expression of CLL1 receptor in MV411 cells as detected by flow cytometry. Black = unstained MV411 cells, blue = CLL1-antibody treated MV411 cells.

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Confocal Microscopy, Staining, Expressing, Flow Cytometry

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: Unmodified PSMA-b-PS NP MV411 are cytocompatible and are taken up in a concentration dependent manner. A) Treatment of MV411 cells with untargeted PSMA-b-PS NPs for 2 and 24 h normalized to the no treatment (NT) group. Two-way ANOVA followed by Dunnett’s multiple comparisons was used to compare NT group with each concentration at all-time points (*p < 0.05). B) Percent of NP positive MV411 cells after treating with increasing concentrations of untargeted PSMA-b-PS NPs. Data represents mean ± standard error mean (N = 3, n = 3). Two-way ANOVA followed by Sidak’s multiple comparisons was used to compare 2 and 24 h time points (****p < 0.0001). C,D) Representative histogram of uptake at 2 and 24 h. E) Median fluorescence intensity (MFI) normalized to no treatment. Data represents mean ± standard (n = 9). Two-way ANOVA followed by Sidak’s multiple comparisons was used to compare 2 and 24 h time points (****p < 0.0001).

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Concentration Assay, Fluorescence

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: Multivalent presentation of cCBP on PSMA-b-PS NPs does not increase NP uptake in MV411 cells. A) Schematic showing cCBP incorporation on NPs. B) Functionalization efficiency of cCBP-NPs. C) Representative transmission electron microscopy (TEM) images of untargeted NP, cCBP200-NP, and CBP30000-NPs. Scale bar = 100 nm. D) Percent of cells positive for cCBP-NPs after incubation for 2 and 24 h in 37 °C. E) Median fluorescent intensity (MFI) normalized to untreated cells of cCBP-NPs after incubation for 2 and 24 h in 37°C. Data represents mean ± standard error mean (n = 9). Two-way ANOVA and Dunnett’s multiple comparisons indicate *p < 0.05 versus untargeted NP.

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Transmission Assay, Electron Microscopy, Incubation

Journal: Advanced biosystems

Article Title: Ligand Density Controls C-Type Lectin-Like Molecule-1 Receptor–Specific Uptake of Polymer Nanoparticles

doi: 10.1002/adbi.202000172

Figure Lengend Snippet: Percent of NP positive MV411 cells is reduced when monoclonal CLL1 antibody is used to block the receptor. A) Percent of NP positive cells after treatment with CLL1-mAb to block prior to NP incubation. B) Percent of NP positive cells after treatment with free cCBP to block prior to NP incubation. Data represents mean ± standard error mean (n = 6). Two-way ANOVA followed by Sidak’s multiple comparisons was used to compare treatment without blocking to CLL1-mab and cCBP groups, respectively (*p < 0.001, ns = not significant).

Article Snippet: Human bi-phenotypic B myelomonocytic leukemia MV411 cells (ATCC, CRL-9591) were maintained at 1 – 5×10 6 cells mL −1 in Iscove’s modified Dulbecco Media (IMDM) supplemented with 10% v/v heat inactivated fetal bovine serum (FBS) and 1% v/v penicillin-streptomycin at 37 °C in 5% CO 2 .

Techniques: Blocking Assay, Incubation